BMG Labtech CLARIOstar Multimode Microplate Reader

CLARIOstar

CLARIOstar – High Performance Microplate Reader with Advanced LVF Monochromators, Spectrometer, and Filters

  • Fluorescence intensity
  • Fluorescence polarization
  • Time-resolved fluorescence
  • FRET
  • Luminescence
  • BRET
  • UV/Vis absorbance
  • Alpha Technology

Anything is possible.

Any Wavelength. Any Bandwidth. Any Assay.

The CLARIOstar is a multimode microplate reader with Advanced LVF Monochromators, highly sensitive filters, and an ultra-fast spectrometer. It is a modular microplate reader, having up to eight different detection modes, including: Fluorescence Intensity, FRET, Fluorescence Polarization, Luminescence, BRET, UV/Vis Absorbance, Time-Resolved Fluorescence, TR-FRET, and AlphaScreen® /AphaLISA®.

Features

Full spectrum UV/Vis absorbance (220 to 1000 nm) with BMG LABTECH’s proprietary ultra-fast spectrometer (

Advanced LVF Monochromator & Filter Selector System

Perfect for measuring and developing fluorescence assays, the CLARIOstar has an advanced monochromator for flexibility that can be used in combination with filters for greater sensitivity. The advanced monochromators, having continually adjustable wavelengths (320 to 850 nm) and bandwidths (8 to 100 nm) for excitation and emission, have significantly increased performance over conventional monochromators.

Description of Technology

BMG’s advanced monochromator system consists of two Linear Variable Filter (LVF) Monochromators & Filter Selectors, one for excitation and one for emission. In addition, a Linear Variable Dichroic Mirror (LVDM) slide is used to separate the excitation from the emission light. The LVF Monochromator consists of a Linear Variable Long Pass (LVLP) slide and a Linear Variable Short Pass (LVSP) slide. The LVLP forms the rising edge while the LVSP forms the falling edge of a bandpass filter. By moving the LVLP and the LVSP slides relative to each other, the system creates a filter with a distinct variable peak wavelength and bandwidth. The high transmission and the outstanding blocking of stray light give the CLARIOstar s monochromator system filter-like performance. In addition, the slides can hold fixed filters, polarizers, and fixed dichroic mirrors for special applications like FP, TR-FRET or Alpha Technology.

LVF-Mono-System

With the CLARIOstar, Anything is Possible – Any Wavelength, Any Bandwidth, Any Assay.

Latest Update

Detection Modes Fluorescence Intensity – including FRET
Fluorescence Polarization / Anisotropy
AlphaScreen® / AlphaLISA®
Luminescence (flash and glow) – including BRET
Time-Resolved Fluorescence – including TR-FRET
UV/Vis Absorbance Spectra
Measurement Modes Top and Bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Spectral Scanning (Fluorescence, Luminescence, Absorbance)
Ratiometric measurements
Well Scanning
Certified for HTRF®
Transcreener® far red FP
Transcreener® red FI
DLReady™
Microplate Formats 6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier Robot compatible
Light Sources High energy xenon flash lamp
Dedicated Laser for AlphaScreen® / AlphaLISA®
Detectors Low noise photomultiplier tube
CCD spectrometer
Wavelength Selection Dual Linear Variable Filter (LVF) MonochromatorsTM
One each for Ex & Em
Linear Variable Dichroic Mirror
Separates Ex & Em LVF Monochromators
Optical Filters
Ex and Em slides hold 4 filters each
LVF Monochromators + Optical Filters
Use one for Ex and the other for Em
UV/Vis Absorbance Spectrometer
Full spectra or 8 distinct wavelengths in < 1 sec / well
Optical Filters Excitation and emission slides for 4 filters each
Optical Path Guides Top and Bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment Automatic focal height adjustment (0.1 mm resolution)
Spectral Range Filters
240 – 750 nm or 240 – 900 nm for FI, FP, TRF
240 – 750 nm for LUM
LVF MonochromatorsTM
320 – 850 nm for FI
320 – 750 nm for LUM
Linear Variable Dichroic
340 – 740 nm for FI, LUM
Spectrometer
220 – 1000 nm for ABS
Sensitivity* FI Filters (top)
< 0.4 pM (< 8 amol/well Fluorescein, 384sv, 20 μL)
FI Filters (bottom)
< 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 μL)
FI Monochromator (top)
< 0.5 pM (< 10 amol/well Fluorescein, 384sv, 20 μL)
FI Monochromator (bottom)
< 3.0 pM (< 150 amol/well Fluorescein, 384g, 50 μL)
FP
< 0.7 mP SD at 1 nM Fluorescein (384sv, 20 μL)
HTRF® (black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 880 % (High Calibrator)
Delta F > 30 % (Low Calibrator)
TRF
< 40 fM (< 0.8 amol/well Europium, 384sv, 20 μL)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL)
AlphaScreen® with Laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS with Spectrometer
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 – 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Read Times Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Reagent Injection Up to 2 built-in reagent injectors
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing
Shaking Linear, orbital, and double-orbital with user-definable time and speed
Incubation +3°C above ambient up to 45°C or 65°C
Software Integrated fluorophore library
Multi-user Reader Control and MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant
Dimensions Width: 45 cm, depth: 51, height: 40; weight: 32 kg
Accessories
Optional Accessories
LVis Plate Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy
Dimensions: Conforms to SBS standards for microplates.
Stacker Plate handler for up to 50 microplates – continuous loading feature
THERMOstar Microplate Incubator and Shaker
Optical Filters Optimized for dyes, fluorophores and specific assays
Filters for all applications from UV to NIR
Customized filters available upon request
Upgrades Upgrades to include options such as additional detection modes, reagent injectors, etc. are available. Please contact your local representative for more information.
* LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplatets: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates

Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.